Subsite Structure of Maltogenic Amylase from Thermomonospora viridis TF-35

We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A -3, A -2, (A -1+A +1), A +2 and A +3 were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol-1, respectively.