Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

Early diagnosis of pine wood nematode(PWN) is a key measure to prevent and control pine wood nematode. To detect PWN in the pine trees early and quickly, the technology of twig dissection with the aid of portable microscope was invented, which could diagnose pine wood nematode quickly. The method was as follows: cutting pine twigs with pruning shears, slicing the twigs up with scalpel, then examining them with the portable microscope. The results showed that the detection rate of nematode was 70% in the infected twigs, 100% in the dying twigs, 60% in partial dead twigs and naught in healthy twigs. The average time for detecting a sample in pine forests was 2.5 minutes. Therefore, the technology of twig dissection with the aid of portable microscope could diagnose pine wood nematode quickly. [Ch, 3 tab. 25 ref.]