THE MASS OF CELLULAR RETINOIC ACID BINDING PROTEIN I INVESTIGATED BY 13 C DEPLETION AND MASS SPECTROMETRY

The accuracy of mass spectrometry used to determine large molecular mass as proteins is often influenced by the isotopic compositions within a protein. Isotopic depletion is a powerful tool to resolve this problem. Using Fourier transform ion cyclotron resonance mass spectrometer, we investigated the 13 C depleted cellular retinoic acid binding protein I, the measured mass accuracy is increased compared with the natural protein KEY WORDS : Molecular mass, Mass accuracy, Isotopic depletion, Monoisotopic Bull. Chem. Soc. Ethiop. 2009 , 23(1), 101-103.