In vitro efficacy of fungicides and bioagents against Fusarium oxysporium f.sp. cubense

The three fungicides, bavistin, nativo and amistar-top in three different concentrations (100, 150 & 200 ppm) and three biocontrol agent Trichoderma viride, T. asperellum and Pseudomonas fluorescens were evaluated against mycelia growth of the causal pathogen Fusarium oxysporum f.sp. cubense in vitro by poisoned food and dual culture techniques, respectively. Observations were taken at 2 days intervals up to 9th days for the assessment of their inhibitory effects. Among them, novel fungicide nativo with all concentrations were found to be most effective against the pathogen followed by bavistin and nativo 100, 150 and 200 ppm inhibition 87.5, 87.8 and 93.9%, respectively after 9th days of treatment. The bio-agents Trichoderma viride and T. asperellum in dual culture with pathogen completely inhibited its mycelial growth in comparison to P. fluorescens up to 9th days. The pathogen fungus continued to increase up to 9th days showing a radial growth of 90 mm in control but in dual culture, it showed growth of 17.60, 18.50 and 27.70 mm in Trichoderma viride, T. asperellum and P. fluorescens, respectively.