High-Sensitive Chemiluminescent ELISA Method Investigation for the Determination of Deoxynivalenol in Rice

In this research, a reliable and rapid chemiluminescent ELISA (CL-ELISA) determination procedure was developed and validated for the detection of deoxynivalenol (DON) in rice. With the help of chemiluminescent substrate, this developed protocol exhibited a high-sensitive character with a limit of detection (LOD) value of 0.94 ng/mL while it was 6.12 ng/mL in our previous report with the conventional colorimetric ELISA procedure by using the same monoclonal antibody (mAb). The mAb used in this research was proved to tolerate no more than 20 % methanol. Therefore, after the extraction procedure with 20 % methanol, samples could be proceeded to take the detection steps directly without dilution by PBS. In spiked rice samples, mean recoveries ranged from 91.40 to 93.48 % with intra-day and inter-day relative standard deviation (RSD) less than 10.62 and 12.41 %, respectively. The results demonstrated that this developed high-sensitive CL-ELISA immunoassay was suitable for screening of DON in rice samples.