Application of pyrosequencing for Salmonella enterica rapid identification.

Abstract Application of pyrosequencing of six Salmonella -specific genes as a rapid Salmonella identification method was tested. Primers for hns , hisJ and hilA had non-specific reactions with non- Salmonella strains. Primers for invA , iroB and fimY had specific PCR products and pyrosequences of Salmonella , suggesting that they were suitable for Salmonella rapid identification.