Determination of S-propargylcysteine by HPLC and Its in situ Intestine Absorption in Rats

An HPLC method was established for the determination of S-propargylcysteine(SPRC) in the intestinal recirculating perfusate.Phenolsulfonphthalein(PP) was determined simultaneously for volumn correction.A C18 column was used with the mobile phase of acetonitrile-water by gradient elution at the detection wavelengths of 220 and 362 nm.The calibration curves of SPRC and PP were linear in the range of 12.5-800 μg/ml and 0.63-40 μg/ml.The rat in situ perfusion trial was applied for the investigation of the absorption mechanism of SPRC.The results indicated that absorption rate constant(Ka) and apparent permeability coefficient(Papp) of SPRC showed a significant difference(P0.05) in different pH perfusion solutions.