Determination of 18 Types of Amino Acids in the Serum of Ischemic Stroke Patients by High Performance Liquid Chromatography-diode Array Detector Derivatized with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate

In this study, we develop a fast and convenient method for the detection of 18 types of free amino acids in the serum of ischemic stroke patients. We use high performance liquid chromatography-diode array detector and thermo C18 column to separate 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate-derivatized amino acids. We find that Gln, His, Gly, Arg, and Cys levels are significantly lower in the serum of ischemic stroke patients comparing with normal persons. Our data indicate that this simple method can be used to detect free amino acids in serum with accurate and reliable results. This study provides a basis for the clinical treatment of ischemic stroke patients and suggests that amino acids can be supplemented to the patients during clinical treatment, which may improve patient prognosis.