Antibacterial activity, cytocompatibility and effect of Bio‐C Temp bioceramic intracanal medicament on osteoblast biology

AIM To analyze the antimicrobial and biological properties of a new bioceramic intracanal medicament (Bio-C Temp), and to compare it with two calcium hydroxide-based intracanal medicaments (Calen® and UltraCal® XS). METHODOLOGY The direct contact and the crystal violet tests were performed to assess the antimicrobial activity of intracanal medicaments against Enterococcus faecalis. The cytocompatibility and the effect of the medication on the biology of the human osteoblast-like cell line (Saos-2) were evaluated with methylthiazole tetrazolium (MTT), neutral red, alkaline phosphatase activity and mineralization (alizarin red) assays. The data were analyzed using one-way ANOVA and Tukey tests, two-way ANOVA and Bonferroni tests, or Kruskal-Wallis and Dunn tests (α = 0.05). RESULTS Bio-C Temp had significantly less antibacterial activity and biofilm biomass reduction than the other intracanal medicaments (P 0.05). CONCLUSIONS Bio-C Temp had similar cytocompatibility at higher dilutions, and higher or similar induction of ALP activity and deposition of mineralized nodules in comparison with Calen® and UltraCal® XS. However, it had significantly less antibacterial and antibiofilm activity than Calen® and UltraCal® XS.