Quantitative evaluation method for cigarette smoke induced cell DNA damage

Belonging to the technical field of toxicological evaluation of cigarette smoke genotoxicity, the invention relates to a quantitative evaluation method for cigarette smoke induced cell DNA damage. The method is characterized by being a method for quantitative determination of cigarette smoke induced cell DNA damage marker phosphorylated histone (gammaH2AX) on the basis of quantum dot labeled fluorescence immunoassay. The method specifically comprises the steps of: (1) cell culture; (2) cigarette smoke exposure; (3) quantitative determination of gammaH2AX in cells on the basis of quantum dot labeled fluorescence immunoassay; and (4) data processing. By means of cell fixation and permeabilization, direct and high-throughput detection of cell samples can be realized, the protein extraction step is omitted, and the detection speed is improved, simultaneous detection of 96 samples can be realized, and the method has the advantage of high throughput. By determining the fluorescence signal of total H2AX in cells, the signal bias caused by differences of cell numbers in each detection hole can be eliminated, the bias of parallel sample fluorescence detection signals can be reduced, and the reproducibility of the method is improved.