A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

Tejia Zhang,Shili Chen,Ismail Syed,Marcus Ståhlman,Matthew J. Kolar,Edwin A. Homan,Qian Chu,Ulf Smith,Jan Borén,Barbara B. Kahn,Alan Saghatelian

A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids

2016

Tejia Zhang
Shili Chen
Ismail Syed
Marcus Ståhlman
Matthew J. Kolar
Edwin A. Homan
Qian Chu
Ulf Smith
Jan Borén
Barbara B. Kahn
Alan Saghatelian

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biological lipids. This protocol describes their extraction from serum and tissue samples, followed by enrichment and analysis by LC-MS.

Keywords:

Bioanalysis
Polyunsaturated fatty acid
Biology
Liquid chromatography–mass spectrometry
Chromatography liquid
Biochemistry
Mass spectrometry
Fatty acid

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