ISOLATION AND IDENTIFICATION OF ARSENIC ELIMINATING BACTERIA FROM LAKE MAHARLOO AND EVALUATION OF THE PATTERN OF THEIR ANTIBIOTIC RESISTANCE
2013
Introduction: Arsenic is one of the most dangerous heavy metals
in the environment that is harmful for organisms such as human
beings. Due to this treat economic approach for elimination of
arsenic from water and wastewater is necessary. In this research
arsenic-resistant bacteria from water and sediments of Lake
Maharloo were isolated and identified then their antibiotic
resistance was evaluated.
Methods and Materials: Sampling was done from water and
sediments of four stations in Maharloo Lake in three seasons.
Bacteria were isolated and purified after cultivation and enriching
samples in LB broth medium containing 5mg/L arsenic oxide.
Isolated bacteria were identified by usual and standard
microbiological tests. Then antibiotic sensitivity was determined by
antibiogram method and Muller Hinton Agar culture medium.
Results: Bacteria such as Bacillus sp, Vibrio sp, Staphylococcus sp,
Corynebacterium sp, Micrococcus sp, Pseudomonas sp and E .coli
were isolated in different seasons from water and sediments of Lake
Maharloo. The maximum and minimum abundance percentage of
arsenic-resistant bacteria was found in sediments of spring
(56.25%) and winter (12.50%) (P<0.05) respectively. Also The
maximum and minimum abundance percentage of arsenic-resistant
bacteria in total of water and sediment related to Khoshk river in
winter and the middle of lake in autumn (P<0.05) respectively.
Antibiotics resistance patterns evaluation revealed that isolated
bacteria had the most antibiotic resistance to penicillin and the
lowest to amikacin in all seasons.
Conclusions: In this researchindigenous arsenic resistant bacteria
of Lake Maharloo were identified. Entrance of arsenic metal and
different antibiotics to Lake Maharloo have caused an increase in
the arsenic resistant bacteria and their antibiotic resistance.
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