Biomesogenic (pre)ordering phenomena and matrix reactions of nucleosides on polyuridylic acid templates studied by scanning force microscopy

Bifunctionally reactive nucleoside and distant nucleoside analogues-adenosine (Ado), S-[(adenine-9-yl)methoxyethyl]-L-cysteine sodium salt (cysA) and 9-vinyladenine (vA) - assemble on complementary polyuridylic acid templates to form complex lyomesophases. Mesophase-derived scanning force microscopy (SFM) elucidations reveal geometries ranging from rod- and toroid-like (cysA) to prevailing toroid-like spheric arrangements (Ado, vA). The dynamics of (U) n .n(vA) self-organizations in hybridization, de- and renaturation are followed up by SFM. The biomesogenic preordering behaviour suggests decisive roles in matrix-directed polycondensations and polymerizations of nucleic acid monomer constituents on assembling polynucleotide templates. Orientating γ-polymerizations yield (U) n matrix-informed 2(U) n .(vA) n arrangements that link the formerly almost discrete toroids into more chromosomal-like patterns. The SFM investigations are assisted by polarizing microscopy and molecular modelling.