Proximity labeling: An emerging tool for probing in planta molecular interactions

Abstract Protein-protein interactions (PPIs) network are key in nearly all aspects of cellular activities. Therefore, identification of the PPIs is important to understand a specific biological process in an organism. In comparison to the conventional methods to probe the PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach to characterize PPIs. However, the application of PL in planta remains at infancy. Here, we summarize the recent progress of the PL approach and its potential utilization to the plant biology field. We specifically summarize advancements of PL, including development and comparison of different PL enzymes, application of PL for dissecting various molecular interactions in different organisms with an emphasis in plant systems.