Simultaneous Determination of Phenolic Glycosides in Curculigins Rhizoma by HPLC

OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of phenolic glycosides in Curculigins Rhizoma.METHODS Angilent 1100 HPLC on a ZORBAX SB C18 column(4.6 mm×250 mm,5 μm) with Extend C18 guard column(4.6 mm×12.5 mm,5 μm) were used.The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid aqueous(B).The gradient elution program was as follows: 0-25 min,4%-6%A;25-58 min,6%-17%A;58-85 min,17%-22%A.The flow rate was kept at 1 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 230 nm.RESULTS The concentrations and peak areas of the 8 phenolic glycosides including 5-hydroxymethylfurfural(1),2-hydroxy-5-(2-hydroxyethyl)phenyl-β-D-glucopyranoside(2),anacardoside(3),orcinol glucoside(4),orcinol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(5),2,6-dimethoxybenzoic acid(6),curculigoside(7) and curculigine A(8) were in good linear relationship.The average recoveries of the 8 constituents met the requirement for determination.CONCLUSION The method is simple,sensitive,reproductive,and suitable for simultaneous determination of a variety of phenolic glycosides in Curculigins Rhizoma.