Transcriptomic analysis reveal differential gene expressions of Escherichia coli O157:H7 under ultrasonic stress

Abstract In order to uncover the molecular regulatory mechanisms underlying the phenotypes, the overall regulation of genes at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation were investigated by RNA-sequencing and real-time quantitative polymerase chain reaction. The results revealed that differential expressions of 1217 genes were significant when exposed at 6.67 W/mL power ultrasonic density for 25 min, including 621 up-regulated and 596 down-regulated genes. Gene transcription related to a series of crucial biomolecular processes were influenced by the ultrasonic stimulation, including carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription and translation. The most enriched pathways were further analyzed in each category. Specifically, genes encoded citrate cycle were down-regulated in E. coli O157:H7, indicating the capacity to decompose carbohydrate and produce energy were decreased under ultrasonic stress. Accompanied with energy loss, the membrane function was affected by the ultrasonic stimulation since the majority of genes encoded ATP-binding cassette transporters were down-regulated. Besides, the autoinducer 2-mediated signal transduction was also inhibited. The interesting thing, however, the protein translation processing was benefited under ultrasonic field. This phenomenon might due to the desperate need of stress response proteins when the bacteria were under stress. We believed that the sonomechanical and sonochemical effects generated by acoustic cavitation were responsible for those gene expression changes.