Rapid determination of mangiferin and neomangiferin in Anemarrhena asphodeloides Bge.by capillary zone electrophoresis with UV detection

Objective:A rapid capillary zone electrophoresis method was,for the first time,developed for the simul- taneous determination of mangiferin and neomangiferin in the Chinese herbal extract from Artemarrhena asphode- loides Bge.Methods:Optimum separation was achieved with 30 mmoL·L~(-1)Borax at pH 9.18.The applied voltage was 25 kV and the capillary temperature was kept constant at 30℃ and the detected wavelength was 214 nm. Uncoated fused silica capillary column 50μm×50 cm(effective length 41.5 cm).Cinnamic acid was used as the internal standard.Results:Regression equation revealed good linear relationship(correlation coefficient:0.9995 and 0.9994)between the ratio of peak area of each compound(mangiferin and neomangiferin)and its concentration (concentration range:8.1-162.0,5.9-118.0μg·mL~(-1)).The relative standard deviations of relative peak area were less than 4.1%.Conclusion:The contents of the two flavonoids in Anemarrhena asphodeloides Bge.were suc- cessfully determined in 6 min,with satisfactory recovery and repeatability.