Study on the Quantification of Macroautoradiography

The relationship between photographic density with radioactivity of particle emitters and exposure period was studied for quantification of macroautoradiography. Photographic density was recorded over 3 different types of X-ray high sensitive films with time by contacting with 7 different radioactivity sheets of autoradiographic standard kits, 14C-polymethacrylate. A good correlation was obtained between radioactivity and photographic density for during a 2 or 3 day exposure period. There was also a good relationship between exposure period and photographic density in case of low radioactivity of 0.025-0.75 μCi/g in the sheets. This relationship was disturbed by unequivalent combination of the radioactivity and exposure period. Discussion was presented to clarify the analysis of such a complex relations as mentioned above.