Adsorption of faecal sterols by crude humin and influence factors

By volume oscillation,migration of fecal steroid between crude humin and water were imitated,the adsorption of fecal steroid in crude humin was studied,and the influence to adsorption of fecal steroid in crude humin with temperature,salinity and pH were observed.It was shown that the increase of temperature has the negatively affected the proceeding of adsorption and increased solubility of fecal sterols releasing the hydrophilic groups in crude humin into water.And that the values of pH have a little effect to the adsorption in the range of acid and base and at the point of 10.0 the adsorption percentage has minimum.And the increase of salt values was beneficial to the adsorption of fecal steroid.It was showed in the orthogonal experiments that the sequence of environmental factor influence to adsorption was as follows: temperaturepHsalt.And also showed that all of the temperature,salinity and pH have a little influence on the ratio values of 5β/(5β+5α)-C27,Coprostanol/24-ethylcoprostanol,coprostanol/cholesterol,24-ethyl coprostanol/sitosterol and 5β/(5β+5α)-C29.And all of them could be used as the indicators of origin of feces.