Extraction and Proteins Removing of Bletilla Striata Polysaccharide

Objective To investigate the extraction methods and content of polysaccharide in Bletilla striata(Thunb)Reichb.f.and to lay the foundation for polysaccharide exploitation and utilization.Methods Bletilla striata crude polysaccharide was extracted by water extraction and alcohol precipitation method.The protein was removeed from the crude polysaccharide by trichloroacetic acid.The contents of polysaccharide and protein were determined by phenol-sulphuric acid method and Bradford method respectively.Results The extraction ratio of Bletilla striata was 34.70%.The polysaccharide concentration of Bletilla striata crude polysaccharide was 67.61%.The ratio of protein removing was 75.86% and the ratio of polysaccharide loss was 0.015%.Conclusion There is a lot of polysaccharide in Bletilla striata and it is worthy of research and development deeply.