Detection of Glomeruli in the Human Cerebellum Using an Immunocytochemical Reaction for Synaptophysin and Confocal Laser Microscopy

The aim of the present work was to develop methods for the detection of complex synaptic groups (glomeruli) in the human cerebellum using an immunocytochemical reaction for synaptophysin. The protocols presented here yield high-quality preparations for light and confocal laser microscopy on which individual glomeruli and intraglomerular axon terminals can be clearly identified. These preparations are suitable for quantitative analysis.