Relative affinities of nucleotide substrates for the yeast tRNA gene transcription complex.

: Apparent Michaelis constants for nucleotides in transcription of yeast tRNA gene by homologous RNA polymerase III with auxiliary protein factors, were found to be remarkably higher in initiation than in elongation of RNA chain. This supports presumptions regarding topological similarities between catalytic centers of bacterial and eukaryotic RNA polymerases.