Analysis and evaluation of penicillin production by using soil fungi

Abstract Penicillin is an antibiotic drug effective against many serious diseases such as syphilis and Staphylococcus infections. Different kinds of fungi have used to obtain various biotechnological products. The current study elucidates the qualitative and quantitative analysis of Penicillin derived from soil fungi using rapid High-performance liquid chromatography (HPLC) when procreating on different growth conditions. The samples are collected and render with HPLC after penicillin produced in both synthetic and semi-synthetic media, over a regular period of incubation. A significant effect on penicillin production has seen in nutrient media or growth media. Our results strongly indicate that the synthetic medium, used the nutrient source as a pure chemical source is good and consistent in penicillin production from 4 to 5 days of incubation. However, systematic, undefined, and rather cheap potato dextrose agar medium is low in penicillin productivity. Penicillin antimicrobial activity was tested on the normal flora of human intestine by taking E. coli, Enterococcus, and Bacillus as indicator organisms and the research results suggest that Penicillin is a potent antibiotic even against the helpful normal flora of the human intestine.