A new fluorescence method for specific determination of tedizolid in real human plasma: Based on twisted intramolecular charge transfer (TICT) blocking

Abstract A distinctive fluorescence method for specific and sensitive determination of tedizolid (a novel oxazolidinone antibacterial) was designed. The presented method relies on fluorescence quencher-removal strategy, which turns the weak fluorescence signal of tedizolid into a stronger one, in acidic ethanolic system (pH 3.5), due to suppression of the intramolecular twisted internal charge transfer (TICT) process. Allowing its determination in bio-fluids and commercial dosage form without any interference at 395 nm after excitation at 300 nm, for the first attempt, the detection and quantification limits were 5.27 and 15.97 ( n g m L - 1 ) , respectively over a concentration range of 25.0–800.0 n g m L - 1 . Moreover, the high sensitivity of the developed method permits its clinical application for efficient and direct determination of the studied drug in biological fluids with good recovery (90.63 ± 1.84%). Additionally, facile application of our intended method paves the way for application in quality control laboratories without sample pretreatment or exhausting extraction steps.