Determination of Glycogen Levels in Human Lung Cancer and Normal Tissues by Fourier Transform Infrared Spectrometry

Glycogen levels in the carcinoma and normal tissues of human lungs were studied by the FT-IR method using the integrated area ratios of glycogen to protein (A1045/A1545). Further amounts of glycogen and protein contained in the same tissues were measured by an anthrone method and by using a dotMETRIC™ kit, respectively. Ratios of glycogen to protein obtained here were compared with the FT-IR spectroscopic data in relation to abnormality and histological classification of the tumors indicating a good correlation between them. The present results show that the FT-IR method is very reliable and that may be of wide application for studying glycogen levels in various tissues as well as diagnosis purpose.