CORRELATION BETWEEN REACTIVE OXYGEN SPECIES AND HOMOCYSTEINE LEVELS IN NORMAL PREGNANCY

The purpose of this study was to determine and to compare the modifications of homocysteine (Hcy), malondialdehyde (MDA) ceruloplasmin and iron concentrations in the second and third trimester of normal pregnancy. The study was performed on 53 women being under surveillance at the Obstetric Gynecology Clinical Hospital Oradea and divided in the following groups: (1) second trimester of pregnancy (n=16), (2) third trimester of pregnancy (n=17), (3) non-pregnant women (n =20). We measured the homocysteine, MDA and the ceruloplasmin levels, in the venous blood samples collected from all the studied groups. Homocysteine concentrations were determined by an enzymatic method, MDA using a method with thiobarbituric acid and ceruloplasmin using the Ravin method. The results were compared with the non-pregnant control group. Supplementary biochemical determinations such as C Reactive Protein (CRP), iron, uric acid, the enzymatic activities of aspartate transaminase (AST) and alanine transaminase (ALT) were also performed. In the studied groups 1 and 2 the concentration of homocysteine was low compared to the control group. Malondialdehyde and ceruloplasmin levels were high compared with those of the non-pregnant women. The conclusions of our researche were: the pregnant women in the second and third trimester present a high level of oxidative stress and a low level of homocysteine. Rezumat Scopul lucrării a fost determinarea si compararea modificărilor concentraţiei de homocisteină (Hcy), malondialdehidă (MDA), ceruloplasmină precum si a sideremiei in trimestrul II si III de sarcină. Studiul a cuprins 53 de femei de la Spitalul de Obstetrică Ginecologie Oradea, impărţite in trei loturi: lotul 1- gravide in trimestrul II de sarcină (n=16), lotul 2- gravide in trimestrul II de sarcină (n=17), lotul 3-femei sănătoase nongestaţionale (n=20). La grupurile luate in studiu s-a evaluat concentraţia de homocisteină, MDA si ceruloplasmină din ser. Homocisteina a fost determinată printr-o metodă