Comparative Study of Wheat Straw Saccharification Processings

Effective saccharification method and optimum saccharification parameters were obtained by comparing the chemical saccharifying method and enzymatic hydrolysis method on wheat straw saccharification effect.The results show that acid hydrolysis saccharification is more efficiency than alkali hydrolysis,and hydrochloric acid saccharifying is the most effective than other reagents in the chemical glycosylation method.The reducing sugar yield is 20.76% when the conditions are hydrochloric acid concentration 25% ,ratio 25∶ 1 mL/g between water and solid mass,and mashing time 5 d.In the enzymatic saccharification process, xylanase saccharifying is more effective than other enzymes.The reducing sugar yield is 33.55% ,when the initial pH 4.5,enzymolysis temperature 40 ℃ ,enzyme/straw 0.06,substrate concentration 0.02 g/mL,and hydrolysis time 24 h.So the effect of xylanase saccharifying is better than the hydrochloric acid glycosylation.