Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors

Aims:  In this study, we identify components of the complement system present in human follicular fluid that affect oocyte development and maturation. Material and Methods:  Using bottom-up liquid chromatography/mass spectrometry/mass spectrometry, we identified complement factors as consistently present in human follicular fluid from 15 different subjects. Results:  According to our gene-chip data, these complement factors are actively produced by granulosa cells. Conclusions:  By applying the computational Ingenuity Pathway Analysis software and database we have identified complement pathways that play a role in oocyte maturation and follicular development.