No detection of macrolide-resistant Mycoplasma pneumoniae from Swedish patients, 1996–2013
2016
Background : Mycoplasma pneumoniae is a common cause of respiratory infections which can cause life-threatening pneumonia and serious extrapulmonary manifestations. Since the year 2000, the emergence of macrolide-resistant M. pneumoniae strains has increased with varying incidences across countries. In China more than 90% of the strains are resistant. M. pneumoniae diagnostics is mostly done with molecular methods, and in Sweden antibiotic resistance surveillance is not routinely performed. The prevalence of macrolide-resistant M. pneumoniae has not previously been studied in Sweden. Material and methods : A total of 563 M. pneumoniae –positive respiratory samples, collected from four counties in Sweden between 1996 and 2013, were screened for mutations associated with macrolide resistance using a duplex FRET real-time PCR method. The real-time PCR targets the 23S rRNA gene, and differentiation between wild-type and resistant strains was achieved with a melting curve analysis. Results : Of the 563 samples included, 548 were analyzed for mutations associated with macrolide resistance. No mutations were found. The detection rate of macrolide-resistant M. pneumoniae in this study was 0% [0.00–0.84%]. Conclusion : No macrolide-resistant M. pneumoniae has been detected in Sweden. However, the emergence and spread of macrolide-resistant M. pneumoniae strains in many countries commands continuous epidemiological surveillance. Keywords: Mycoplasma pneumoniae; antibiotic resistance; macrolide; treatment; diagnostics (Published: 1 June 2016) Citation: Infection Ecology and Epidemiology 2016, 6: 31374 - http://dx.doi.org/10.3402/iee.v6.31374
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
29
References
7
Citations
NaN
KQI