OFD1 and VFL3/CCDC61 in basal body positioning and docking in Paramecium

Objectives Ciliogenesis is conditioned by a correct positioning/ anchoring of the basal body at the cell surface. In Paramecium we have shown that three conserved proteins FOR20, centrin 2 (CEN2) and centrin 3 (CEN3) participates in this process, with FOR20 and CEN2 being also involved in the transition zone assembly. We established a chronology in basal body assembly: CEN2 is required for FOR20 recruitment, the latter being necessary to recruit CEN3. Our goal now is to integrate others molecules in this cascade.