Subsequent Study Regarding some Histopathological Tissue Changes Related to Ethylenediaminetetraacetic Acid (EDTA) Experimental Exposure in Prussian Carp (Carassius Gibelio)

In this paper we present the results obtained following the continuation of a previous experiment, in order to describe more rigorous the degree of morphofunctional tissue damages in Carassius gibelio specimens exposed to EDTA chronic treatment. Healthy prussian carp specimens were collected from a local pond and acclimated for two weeks in laboratory conditions. Acclimated fish were housed in a 60 l capacity glass aquariums (20 fishes/aquarium) and divided into four groups: a control group maintained in EDTA-free freshwater and others three groups receiving increasing doses of EDTA in water. Microscopical examination of the liver and kidney tissue sampled and processed at the end of a 21 days experimental period, shows alterations ranging from mild to medium and severe, proportionally with increasing dose of EDTA incorporated into water.