Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue

Chloride (Cl−) secretion is crucial for intestinal fluid secretion. Therefore, effects of the polyphenol Resveratrol (RSV) on Cl− secretion have been investigated. In a previous study, we observed effects of RSV on forskolin-induced Cl− secretion in the porcine jejunum but not the ileum although RSV itself induced a transepithelial ion current that may represent Cl− secretion in the ileum. The aim of this study was to gain further insights regarding the effects of RSV on characteristics of Cl− secretion in the porcine ileum using the Ussing chamber technique (recording of short circuit currents (Isc) as a measure for epithelial net ion transfer). RSV increased the Isc in the porcine ileum but not in the porcine jejunum as is already known. This increase was absent in a Cl−-free buffer system, indicating that RSV indeed induces Cl− secretion. However, the carbachol-induced Isc was significantly inhibited by RSV indicating an inhibition of Ca2+-induced Cl− secretion. The cellular basis for these contradictory, segment specific results of RSV on Cl− secretion has to be subjected to further studies. The results also underline, that is difficult to generalize effects of RSV between different intestinal locations, organs, cell culture models or species.