IN VITRO DOSE DEPENDENT STUDY ON ANTI HUMAN PATHOGENIC BACTERIAL AND FREE RADICAL SCAVENGING ACTIVITIES OF METHANOLIC SEED COAT EXTRACT OF BORASSUS FLABELLIFER L.

The study aimed to evaluate the anti bacterial activity against human pathogenic organisms and free radical scavenging activities of methanolic seed coat extract of Borassus flabellifer . The anti bacterial activity were tested against human pathogenic bacteria sps like Salmonella typhi, Vibrio cholerae, Shigella dysenteriae, and Enterococcus faecalis which are clinical isolates collected at department of microbiology, King Goerge hospital of Andhra medical college, Visakhapatnam. The methanolic seed coat extract was showed significant inhibitory activity. The highest inhibitory activity was showed against Vibrio cholerae . The MIC of seed coat extract was found to be the range between 0.1-10 mg/ml. 0.1 mg/ml was the lowest MIC to Vibrio cholerae 1, 10 & 10 mg/ml were the MIC values to Shigella dysenteriae, salmonella typhi and Enterococcus faecalis respectively. Radical scavenging activity of methanolic seed coat extract of B. flabellifer was carried out using DPPH, ABTS employing as acorbic acid as standard drug. 2.85, 7.90µg/ml were the IC50 values of seed coat extract to ABTS, DPPH radical scavenging activities respectively. The anti bacterial as well as radical scavenging activities dose dependently increased with seed coat extract. The present study revealed that seed coat of Borassus flabellifer has shown significant anti-bacterial and radical scavenging (anti-oxidant) activities.