[To knockdown survivin gene expression by siRNA in SO-Rb50 cells].

2008 
Objective To investigate cell proliferation and apeptosis status of human retinoblagtoma cell line SO-Rb50 after knockdown of Survivin gene by means of small interfering RNA(siRNA).Methods Survivin specific siRNA designed from the human gene sequence and nonsense siRNA(as a negative control)Waft transfected into SO-Rb50 cells.The inhibition of the expression of Survivin mRNA and protein levels were detected by reverse transcription-polymerage chain reaction(RT-PCR)and Western blot Proliferation inhibition rate of SO-Rb50 cells was analyzed by MTT assay.Apoptotic rate and cell cycle were analyzed by flow cytometry(FCM)and the apoptotic morphology was observed by fluorescent microscope.Results The expression of Survivin at both mRNA and protein level in specific siRNA group decreased significantly in comparison with untransfected group and nonsense siRNA group after 24 hours of transfection.The proliferation of SO-Rb50 was inhibited in the Survivin specific siRNA group at the concentrations of 35,70 and 100 nmoL/L(P<0.05).Flow cytometry showed obvious apoptotic peak in Survivin specific group with an accumulation of cells in the G0/G1 phage and a decrease in G2/M phage and S phase.Typical apoptosis morphyology was also observed under fluorescent microscope.Conclusions Sunrivin specific siRNA could inhibit SO-Rb50 cell proliferation and induced apeptosis by knockdown of Survivin gene.Our data suggests that the use of Survivin-specific siRNA deserves further investigation as a novel approach to retinoblastoma therapy. Key words: Retinoblastoma;  Cell line,tumor;  RNA,small interfering;  Apoptosis; Microtubule-associated protein
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