STIMULATED RAMAN SCATTERING MICROSCOPY IN SINGLE CELL

Taking advantage of Stimulated Raman scattering, a labelfree single cell imaging technique based on vibrational contrast can be implemented. In this work, the successful realization of a nonlinear microscope, not purchasable in commerce, based on Stimulated Raman scattering is described. In order to test the microscope, polystyrene beads images are carried out. Finally, in order to demonstrate the feasibility of Stimulated Raman scattering imaging, preliminary label free images of adipocyte cell are reported.