Study on the characteristic of anti-HLA antibody produced by hemodialysis in renal transplantation patients before operation

Objective To understand the distribution and probability of anti-human leukocyte antigen (anti-HLA) antibody produced by hemodialysis before renal transplantation. Methods A total of 169 serum samples were collected from January 2015 to December 2017, from patients without sensitization history before kidney transplantation in our hospital. Anti-HLA antibodies were detected using Luminex 200 detection platform and corresponding reagents, and then the data was analyzed. Results 64.50% (109/169) of the 169 hemodialysis patients produced anti-HLA antibody. With the prolongation of hemodialysis time, the positive rate of anti-HLA antibody in patients′ serum increased, but the difference was not significant (χ2=6.009, P>0.05). 186 anti-HLA ClassⅠantibody and 158 ClassⅡantibody detected. Among the anti-HLA ClassⅠantibody, the highest frequency antibody were anti-A11, A25, A66, A80, B8, B75, B76, and Cw17. Among anti-HLA ClassⅡ antibody, anti-DR4, DR51, DR52, DR53, DQ2, DQ4, DQ6, DQ7, DP1, DP5, DP11 and DP13 were higher. Conclusions Anti-HLA antibody can be produced by patients with non sensitized renal transplantation after hemodialysis. Hemodialysis may be another factor of sensitization, which should be paid attention to in clinic. Key words: Kidney transplantation; Hemodialysis; Anti-HLA antibody; Sensitization