Amadoriase the anionic detergent-resistant with improved

Even in the presence of a strong surfactant than conventional, it provides a composition capable of measuring glycated hemoglobin. The invention 80 of the amadoriase derived Coniochaeta sp shown in SEQ ID NO: 1, position 71, position 175, position 172, position 279, position 12, 9, 77, 30, 28, 13, 3, 4, 286, 204 positions, 338, 44, 340-position and 194-position one or more amino acid positions corresponding to positions selected from the group consisting of is substituted amadoriase, and anionic surfactants also provides a glycated hemoglobin measuring composition comprising amadoriase activity remains in the presence of. It can provide enzymes and glycohemoglobin measuring composition excellent in stability even when exposed to anionic surfactants by the invention.