TheXbaI-BlnI-CeuI GenomicCleavage Map ofSalmonella paratyphi B

TnlOtransposons inserted in 61different genesofS.typhimurium LT2weretransduced byuseofbacteriophage P22into S.paratyphi B.The locations ofTnlOinsertions on thechromosome ofS.paratyphi Bwere determined byuse ofXbalandBlnIsites inTnlO,revealing thepositions ofgeneswithTnlOinsertions inS.paratyphi B.AllsevenCeuIsites (inrrl genes for23SrRNA)andmostoftheXbaIandBlnIsites inrrngenesforGlt-tRNA areconserved, butonlyabouthalf oftheXaI andBlnIsites outside rrngenesareconserved. Geneorder isidentical inthe68genesthat we could compare between S.paratyphi B andS.typhimurium LT2,andthelengths ofintervals between thegenes are often thesame,butthere areseveral instances ofdifferences ininterval lengths, indicating thatinsertions or