Isolation of major Phytoconstituents and Standardization of different extracts of Boerhavia diffusa by RP-HPLC

Nature is the basketful of medicinal herbs, which consist of plethora of bio-active constituents. Natural products isolated from the plant source have been an interim part in prevention and curing of many diseases since from the era of human civilization. Numerous of natural products such as Acetylsalicyclic acid, Salicin, Morphine, Digitoxin, Quinine, Vicrestine, Vinblastine, Podophyllotoxin, and Pilocarpine have been proved to be consecrated in health care sector. Due to severe toxic effects, unavoidable side effects, and inadequate availability of medicines, World Health Organization (WHO) promotes heavily the practice of herbal medicines. So as to address this issue, a retrospective research may be required to the exploration of the bioactive constituents from the herbal sources. One such common plant is Boerhaavia diffusa belongs to family Nyctaginaceae has been explored by many research articles with a wide prospective angle to its ethnopharmacological significance, phytoconstituents, and its biological activities. The ambition of the current research was to enlighten the standardization of the different alcoholic extracts with isolated phytomarkers such as Kaempferol, Quercetin, and Boeravinone B. The standardization of the extracts was carried by reverse phase high performance liquid chromatography (RP-HPLC). The chromatograms were developed by gradient elution with 0. 05% TFA in acetonitrile as mobile phase. The samples were detected by UV detector at 340 nm. This conclusive research finds the different percentage of phytomarkers in various alcoholic extracts Boerhaavia diffusa.