Estudio de 5 compuestos orgánicos volátiles en aire exhalado en la enfermedad pulmonar obstructiva crónica
2017
espanolIntroduccion Un factor de riesgo importante para el desarrollo de la enfermedad pulmonar obstructiva cronica (EPOC) es el humo del tabaco, que genera estres oxidativo en las vias respiratorias, dando lugar a la produccion de compuestos organicos volatiles (VOC). El objetivo del trabajo es su identificacion en el aire exhalado y su posible utilidad como biomarcadores de la enfermedad. Metodo Se analizo el aire exhalado de 100 voluntarios sanos, clasificados en 3grupos (no fumadores, exfumadores y fumadores activos) y un grupo de 57 pacientes con EPOC. La muestra de aire exhalado se recogio mediante BioVOC® y se traspaso a tubos de desorcion para su posterior analisis por cromatografia de gases y espectrometria de masas. Los VOC analizados fueron aldehidos lineales y acidos carboxilicos. Resultados Hexanal mostro diferencias estadisticamente significativas entre el grupo EPOC y los controles sanos (no fumadores y exfumadores), y nonanal entre el grupo control no fumador y el grupo EPOC. Conclusiones Hexanal discrimina entre pacientes con EPOC y controles sanos no fumadores y exfumadores. Nonanal diferencia entre fumadores y exfumadores (con o sin EPOC) frente a controles no fumadores. EnglishIntroduction A major risk factor for chronic obstructive pulmonary disease (COPD) is tobacco smoke, which generates oxidative stress in airways, resulting in the production of volatile organic compounds (VOC). The purpose of this study was to identify VOCs in exhaled breath and to determine their possible use as disease biomarkers. Method Exhaled breath from 100 healthy volunteers, divided into 3groups (never smokers, former smokers and active smokers) and exhaled breath from 57 COPD patients were analyzed. Samples were collected using BioVOC® devices and transferred to universal desorption tubes. Compounds were analyzed by thermal desorption, gas chromatography and mass spectrometry. VOCs analyzed were linear aldehydesand carboxylic acids. Results The COPD group and healthy controls (never smokers and former smokers) showed statistically significant differences in hexanal concentrations, and never smokers and the COPD group showed statistically significant differences in nonanal concentrations. Conclusions Hexanal discriminates between COPD patients and healthy non-smoking controls. Nonanal discriminates between smokers and former smokers (with and without COPD) and never smokers.
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