Effect of Optimum and Suboptimum Dietary Protein on Hemolymph Physiology, Oxidative Physiology, Plasma Fatty Acids, and Histoarchitectural Modulations in Farmed Giant Freshwater Prawns Macrobrachium rosenbergii

AbstractWe examined the effect of optimum (32%) and suboptimum (27%) levels of dietary protein on the hemolymph and oxidative physiology of giant freshwater prawn Macrobrachium rosenbergii juveniles. Wild juveniles of different stocks were collected from the west coast (Gujarat [G-stock] and Maharashtra [M-stock]) and east coast (Andhra Pradesh [A-stock]) of India. Prawns were raised in 200-m2 culture ponds, with individual animals tracked by using stock-specific, colored elastomer tags. Hemolymph glucose, plasma total protein, albumin-to-globulin (A:G) ratio, plasma calcium, and activity of five key metabolic enzymes in plasma (plasma cholinesterase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, and amylase) were examined to compare the hemolymph physiology due to dietary protein variations fed to the different stocks. Plasma fatty acid profile was also monitored. Effect of feeding on oxidative physiology of prawns was analyzed by measuring the catalase and acetylcholinesterase...