In vitro response of GA3 in caulogenesis of Fiver nut

A protocol was optimized for the caulogenesis of fiver nut. Internodal explant showed immediate response in shoot regeneration and production of callus in in vitro cultures of Caesalpinia bonducella (L.) Roxb. commonly known as fiver nut. MS medium supplemented with 1 to 10 mg/l GA 3 was found to induce callus. The Internodal explant inoculated on MS medium with 6 mg/l GA3 was found to produce shoots after 35 days of inoculation. Maximum amount of pale yellow coloured friable callus was produced in 7mg/l GA3 of dry weight 1.513± 0.108 g. The method can be used to generate callus and shoot which are natural sources of pharmaceutical Compounds without disturbing the natural population of the plant.