Age and sex differences in the innate defensive behaviors of C57BL/6 mice exhibited in a fear conditioning paradigm and upon exposure to a predatory odor

Abstract Defensive responses of mice include an array of specific behaviors that involve adaptive components based on the assessment of the threat of confrontation. The freezing response is represented by a motionless moment without any specific posture or behavioral sequence, and it is widely used in the fear conditioning paradigms and other relevant defensive situations. However, freezing measurements include fragmental components of several defensive behaviors that are exhibited during the session, such as behavioral inhibition, crouching, and a moment of risk assessment behavior. From an ethological view, behavioral analyses of C57BL/6 mice of both sexes and three different ages (postnatal days (P) 25, 35, and 65) revealed a rich variety of defensive behaviors during a fear conditioning session and in response to predatory odor exposure as a nonconditioned behavior. P-25 and 35 mice exhibited more behavioral inhibition than P-65 adult mice, and P-65 mice exhibited a crouching posture more often than younger mice. This age difference was more pronounced in males. The stretch-attend posture (SAP) increased with age, except in P-25 males, which exhibited robust SAP in response to a conditioned cue; this response indicates that P-25 males are defensive in a situation-nonmatching manner. Situation-dependent defense strategies were revealed in P-35 and 65 mice: Fear conditioning paradigm induced more robust defensive responses than predatory odor exposure, to which mice primarily exhibited SAP. A sex-based difference was revealed in adult mice. Males tended to show more passive defensive responses, such as crouching and withdrawal, and females exhibited more active responses, such as SAP. These age- and sex-based differences may stem from the ethological demands and illustrate adolescent ontogenetic processes of defense behavior.