自动化制备多巴胺转运蛋白显像剂11C-β-CFT及其在正常新生猪脑内PET/CT显像中的应用

Objective To develop an automatic synthesis method for the on-line preparation of dopamine transporter imaging agent 11C-methyl-N-2-β-carbomethoxy-3-β-(4-fluorophenyl)-tropanel (11C-β-CFT), and to study the regional brain DAT uptake in healthy newborn piglets using PET/CT. Methods 11C-β-CFT was automatively prepared on-line using TRACER- lab FX-pro system of GE company. Dynamic scans were carried out in 3 healthy newborn piglets using PET/CT. The changes of distribution of 11C-β-CFT in brain with time elapse were observed, and the curve of activity-time was drawn accordingly. Results The radiochemical purity of 11C-β-CFT was over 98%, and the specific radioactivity was more than 370MBq/μmol. The synthesis yield was 20mCi. After injection, 11C-β-CFT accumulation in cortical, basal ganglia, and cerebellum was showed clearly at 5 min. The radioactivity in cortical and cerebellum exhibited decrease with time going. The radioactivity mainly accumulated in basal ganglia areas. Conclusion The automatic on-line preparation method is verified in producing 11C-β-CFT of high radiochemical purity for a short period of time. Some brain receptor research on newborn piglets may be carried out with 11C-β-CFT PET/CT imaging.