The Anti-aging Effects of Various Berries in the Human Skin Keratinocyte (HaCaT) Cells

Su Jung Lee, Hye Ran Choi, Jin-Cheol Lee, Hee Jeon Park, Hee Kwon Lee, Jong Tae Jeong, and Tae-Bum Lee*Gochang Black Raspberry Research InstituteAbstract Ultraviolet B (UV-B) irradiation is a negative factor that induces skin damage, inflammation, and aging. UV-B irradiation induces the inflammatory response through interleukin (IL)-6 and IL-8 expression in keratinocytes. Inaddition, it induces the production of reactive oxygen species (ROS) and the activation of matrix metalloproteinase-1(MMP-1), which plays an important role in collagen 1 degradation in the extracellular matrix. We investigated the anti-aging effects of five kinds of berry in human skin keratinocyte (HaCaT) cells using juice of black raspberry (Rubusoccidentalis), blueberry wild (Vacciniun angustifolium) and cultivar (Vacciniun corymbosum), black chokeberry (Aroniamelanocarpa (Michx.) Elliott), and mulberry (Morus abla). HaCaT cells irradiated with UV-B exhibited increased ROSgeneration, as well as IL-6, IL-8, and MMP-1 gene expression, when compared to the control cells that were not irradiatedwith UV-B. However, pre-treatment of berry juice before UV-B irradiation significantly down-regulated the UV-B-inducedROS generation and inflammatory cytokine and MMP-1 expression. The results suggest that all berries have anti-agingeffects including lowering inflammatory cytokine levels, ROS generation, and MMP-1 expression in HaCaT cells duringUV-B irradiation.Keywords: berry, ultraviolet B, inflammatory cytokine, reactive oxygen species, matrix metalloproteinase-1