Optimization of Protection Condition for Composite Freeze-Dried Starter of Paocai

To obtain the better formula of the biomaterials lyoprotectant for composite starter of Paocai, five lyoprotectants were studied with math method those are orthogonal design, Quadratic polynomial stepwise regression and etc. The results showed, 14.7% of hydrolyzate of skim milk, 1.1% of amount of glucose, 3.4% of amount of glycerol, 5.0% of amount of sodium glutamate, 1.2% of amount of Vc were added to the culture of Lactobacillus. After lyophilized, colonies of freeze-dried starters can reach 3.25×1010 cfu/mL. Response surfaces are drawn according to the mathematical model among factors, and it was discussed the impact of lyoprotectants.