Study on the protective function of DHEA on hematopoietic induced by radiotherapy and chemotherapy

Objective To observe the protective function of dehydroepiandrosterone （DHEA） on hematopoietic in C57BL/6 mouse induced by radiotherapy and chemotherapy. Methods Constructing the animal models with hematopoietic injury by 8.0 Gy 137Csγray and cyclophosphamide respectively. Observe the changes of colony-forming unit-spleen（CFU-S）, populations of peripheral WBC and bone marrow nu-cleated cells and index of spleen in all DHEA treated groups. Results DHEA can reduce injury of 137Csγray and cyclophosphamide induced hematopoietic function injury of C57BL/6 mouse. Compared with the blank control group, the DHEA group can significantly increase the number of peripheral blood leukocyte, bone marrow nucleated cells and CFU-S, and there is statistically difference between DHEA treated group and the blank control group. Index of spleen in the high dose group is higher than that in the blank control group. Conclusion DHEA have protection function on hematopoietic in C57BL/6 mouse induced by ra-diotherapy and chemotherapy. Key words: Gama rays; Cyclophosphamide; Hematopoietic function; Dehydroepiandrosterone