Method for picking up and separating compound of flavone being as effective content of cinquefoil, and application

This invention discloses a method for extracting and separating flavonoid compounds from Potentilla chinesis. The chemical formulae of the flavonoid compounds are shown in formulae 1 and 2. The method comprises: (1) pulverizing Potentilla chinesis, extracting ethanol by refluxing, filtering, incorporating the filtrates, and vacuum-evaporating to obtain an etractum; (2) dissolving the extractum in ethanol, performing polyamide column chromatographic separation, eluting with ethanol/water, collecting the elutes, and vacuum-evaporating to obtain an extractum; (3) performing C-18 column chromatographic separation under normal pressure, eluting with ethanol/water, collecting the elutes, and vacuum-evaporating to obtain the effective components 1 and 2. The flavonoid compounds can be used to manufacture drugs for treating diabetes. The polyamide and C-18 fillers in this invention can be recycled, and water and ethanol as the solvents.