Method for detecting lactic acid bacteria, lactic acid bacteria detection kit, and lactic acid bacteria detection instrument
2016
In the present invention, four types of lactic acid bacteria (Lactobacillus plantarum, brevis, buchneri, and fructivorans) are each simultaneously detected specifically. An amplification reaction by PCR is conducted using DNA extracted from cells contained in a sample, a primer set shown by SEQ ID NO: 1 and SEQ ID NO: 2 that targets an RNA polymerase gene of Lactobacillus plantarum, and a primer set comprising base sequences shown by SEQ ID NO: 3 and SEQ ID NO: 4 that targets the 16SrRNA gene of Lactobacillus plantarum, brevis, buchneri, and fructivorans. The presence of Lactobacillus plantarum, brevis, buchneri, and fructivorans is determined simultaneously based on the amplified product obtained.
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