Образование полиплексов новыми поверхностноактивными гребнеподобными полиамфолитами и плазмидной ДНК

Formation of the interpolyelectrolytic complexes (polyplexes) of plasmid DNA and novel surface active comb-like polyampholytic carriers was studied. To do that, the method of determi ning DNA retardation during its electrophoresis in the agarose gel was used. Optimal conditions for the formation of such polylexes were defined: PC concentration 0.1–0.003 %, рН 7.4, 20 min, 24 °С. It was found that polyampholyte posses sing quaternized amino-containing side chains is capable to form the most stable polyplexes with plasmid DNA. The association of DNA with polyampholytic carriers and its release from such complex do not cause changes in DNA structure. Therefor, the polyampholytic carriers under study protected DNA from its nuclease cleavage. Thus, novel surface active comb-like polyampholytes are perspective carriers for delivering DNA to the recipient cells.